anchorage dependence

anchorage dependence
The necessity for attachment (and spreading) in order that a cell will grow and divide in culture. Loss of anchorage dependence seems to be associated with greater independence from external growth control and is probably one of the best correlates of tumorigenic events in vivo. Anchorage independence is usually detected by cloning cells in soft agarose; only anchorage-independent cells will grow and divide (as they will in suspension).

Dictionary of molecular biology. 2004.

Игры ⚽ Нужно решить контрольную?

Look at other dictionaries:

  • dependence — The quality or condition of relying upon, being influenced by, or being subservient to a person or object reflecting a particular need. [L. dependeo, to hang from] anchorage d. the need of normal cells to have an appropriate surface to attach to… …   Medical dictionary

  • Plating Efficiency — ( PE ) is a measure of the number of colonies originating from single cells. It is a very sensitive test and is often used for determining the nutritional requirements of cells, testing serum lots, measuring the effects of growth factors, and for …   Wikipedia

  • POU4F2 — POU domain, class 4, transcription factor 2, also known as POU4F2, is a human gene.cite web | title = Entrez Gene: POU4F2 POU domain, class 4, transcription factor 2| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene Cmd=ShowDetailView… …   Wikipedia

  • ascites tumour — Tumour that grows in the peritoneal cavity as a suspension of cells. Obviously such cells have lost anchorage dependence, and they can easily be isolated and passaged. Hybridomas are sometimes grown as ascites tumours, and the ascites fluid can… …   Dictionary of molecular biology

  • microcarrier — Microcarriers are small solid or in some cases immiscible liquid spheres, on which cells may be grown in suspension culture. They provide a means of obtaining large yields of cells in small volumes. The cells must exhibit anchorage dependence of… …   Dictionary of molecular biology

  • soft agar — Semi solid agar used to gelate medium for culture of animal cells. Placed in such a medium, over a denser agar layer, the cells are denied access to a solid substratum on which to spread, so that only cells that do not show anchorage dependence… …   Dictionary of molecular biology

  • Political positions of Sarah Palin — right|thumb|250px|Sarah Palin at the annual blessing of the fishing fleet in Dillingham, Alaska. Sarah Palin is the Governor of Alaska and Republican vice presidential candidate for the 2008 United States presidential election.ocial and legal… …   Wikipedia

  • Alaska — This article is about the U.S. state of Alaska. For other uses, see Alaska (disambiguation). Alaskan redirects here. For other uses, see Alaskan (disambiguation). State of Alaska …   Wikipedia

  • Sarah Palin — Sarah Palin …   Wikipedia

  • Mike Gravel — United States Senator from Alaska In office January 3, 1969 – January 3, 1981 Preceded by Ernest Gr …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”